Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples

Publication year: 2011 Source: Research in Veterinary Science, Available online 22 October 2011 D. Escribano, A.M. Gutiérrez, S. Martínez Subiela, F. Tecles, J.J. Cerón The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals

Publication year: 2011 Source: Research in Veterinary Science, Available online 22 October 2011 D. Escribano, A.M. Gutiérrez, S. Martínez Subiela, F. Tecles, J.J. Cerón The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals

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Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples

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