Separation and purification ofToxoplasma gondiitachyzoites fromin vitroandin vivoculture systems

Publication year: 2011 Source: Experimental Parasitology, Available online 15 October 2011 Liang Wu, Sheng-xia Chen, Xu-gan Jiang, Xing-li Fu, Yu-juan Shen, … In this study, we evaluated 4 methods to separate and purifyToxoplasma gondiitachyzoites fromin vivoandin vitroculture systems, including trypsin digestion, purification with a 3-μm filter, CF-11 cellulose purification, and Percoll purification. Our results indicate that both purification with a 3-μm filter and CF11 cellulose purification methods remove leukocytes or HeLa cells, and can therefore be used as candidate methods for the purification ofin vivoandin vitroculture products

Publication year: 2011 Source: Experimental Parasitology, Available online 15 October 2011 Liang Wu, Sheng-xia Chen, Xu-gan Jiang, Xing-li Fu, Yu-juan Shen, … In this study, we evaluated 4 methods to separate and purifyToxoplasma gondiitachyzoites fromin vivoandin vitroculture systems, including trypsin digestion, purification with a 3-μm filter, CF-11 cellulose purification, and Percoll purification. Our results indicate that both purification with a 3-μm filter and CF11 cellulose purification methods remove leukocytes or HeLa cells, and can therefore be used as candidate methods for the purification ofin vivoandin vitroculture products

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Separation and purification ofToxoplasma gondiitachyzoites fromin vitroandin vivoculture systems

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