On the substrate preference of glutaryl acylases

Publication year: 2011 Source: Journal of Molecular Catalysis B: Enzymatic, Available online 9 December 2011 Elena Rosini, Claudia Stella Monelli, Loredano Pollegioni, Sergio Riva, Daniela Monti The substrate preferences of three acylases – two wild-type enzymes and an evolved variant obtained by directed evolution – which are prototypical enzymes for glutaryl-7-ACA acylase and cephalosporin C acylase subfamilies, have been investigated. A preliminary screening of enzymes’ performances on a large set of substrates has been carried out by a colorimetric assay performed in 96-well plates and by a pH-Stat monitoring the hydrolytic activities.

Publication year: 2011 Source: Journal of Molecular Catalysis B: Enzymatic, Available online 9 December 2011 Elena Rosini, Claudia Stella Monelli, Loredano Pollegioni, Sergio Riva, Daniela Monti The substrate preferences of three acylases – two wild-type enzymes and an evolved variant obtained by directed evolution – which are prototypical enzymes for glutaryl-7-ACA acylase and cephalosporin C acylase subfamilies, have been investigated. A preliminary screening of enzymes’ performances on a large set of substrates has been carried out by a colorimetric assay performed in 96-well plates and by a pH-Stat monitoring the hydrolytic activities.

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On the substrate preference of glutaryl acylases