Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues

Publication year: 2011 Source: Archives of Biochemistry and Biophysics, Available online 29 September 2011 Catera L. Wilder, Keon-Young Park, Philip M. Keegan, Manu O. Platt Cathepsins K, L, S, and V are cysteine proteases that have been implicated in tissue-destructive diseases such as atherosclerosis, tumor metastasis, and osteoporosis. Among these four cathepsins are the most powerful human collagenases and elastases, and they share 60% sequence homology

Publication year: 2011 Source: Archives of Biochemistry and Biophysics, Available online 29 September 2011 Catera L. Wilder, Keon-Young Park, Philip M. Keegan, Manu O. Platt Cathepsins K, L, S, and V are cysteine proteases that have been implicated in tissue-destructive diseases such as atherosclerosis, tumor metastasis, and osteoporosis. Among these four cathepsins are the most powerful human collagenases and elastases, and they share 60% sequence homology

Excerpt from:
Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues

Incoming search terms:

  • zymography protocol

Related posts:

  1. Manipulating the proximal triad His-Asn-Arg in human myeloperoxidase
  2. Catalase-Like Activity of Human Methemoglobin: A Kinetic and Mechanistic Study
  3. In vivodifferentiation of adipose-derived stem cells in an injectable poloxamer-octapeptide hybrid hydrogel
  4. Functional interactions between the oxytocin receptor and the β2-adrenergic receptor: Implications for ERK1/2 activation in human myometrial cells
  5. Step activity monitoring to assess ambulation before and after total ankle arthroplasty
  6. Lack of amino acids in mouse hepatocytes in culture induces the selection of preneoplastic cells
  7. The evaluation of cyclic uniaxial strain on myogenic differentiation of adipose-derived stem cells
  8. KATPchannels in mesenchymal stromal stem cells: Strong up-regulation of Kir6.2 subunits upon osteogenic differentiation
  9. The N-terminal ß-barrel domain of mammalian lipoxygenases including mouse 5-lipoxygenase is not essential for catalytic activity and membrane binding…
  10. Solid state dye-sensitized solar cells prepared by infiltrating a molten hole conductor into a mesoporous film at a temperature below 150 °C