Fluorometric estimation of surface associated microbial abundance

Publication year: 2011 Source: Journal of Microbiological Methods, Available online 29 December 2011 Gabriel J. Swenson, Isaac M. Hagenbuch, James L. Pinckney, Richard A. Long Surface associated microbes have historically been difficult to accurately and effectively enumerate. In the current study, we propose a rapid and simple method for estimating abundance of surface associated microbial cells by fluorescence of SYBRGreen stained bacteria andin vivochlorophyllafluorescence of benthic diatoms in 24 and 48-well microtiter plates.

Publication year: 2011 Source: Journal of Microbiological Methods, Available online 29 December 2011 Gabriel J. Swenson, Isaac M. Hagenbuch, James L. Pinckney, Richard A. Long Surface associated microbes have historically been difficult to accurately and effectively enumerate. In the current study, we propose a rapid and simple method for estimating abundance of surface associated microbial cells by fluorescence of SYBRGreen stained bacteria andin vivochlorophyllafluorescence of benthic diatoms in 24 and 48-well microtiter plates.

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Fluorometric estimation of surface associated microbial abundance