Development of a rotor-gene real-time PCR assay for the detection and quantification ofMycoplasma genitalium

Publication year: 2011 Source: Journal of Microbiological Methods, Available online 29 December 2011 Etienne E. Müller, Johanna M.E. Venter, Mahlape P. Magooa, Charles Morrison, David A. Lewis, … We developed and validated a real-time quantitative polymerase chain reaction (qPCR) assay to determineMycoplasma genitaliumbacterial load in endocervical swabs, based on amplification of thepdhDgene which encodes dihydrolipoamide dehydrogenase, using the Rotor-Gene platform.

Publication year: 2011 Source: Journal of Microbiological Methods, Available online 29 December 2011 Etienne E. Müller, Johanna M.E. Venter, Mahlape P. Magooa, Charles Morrison, David A. Lewis, … We developed and validated a real-time quantitative polymerase chain reaction (qPCR) assay to determineMycoplasma genitaliumbacterial load in endocervical swabs, based on amplification of thepdhDgene which encodes dihydrolipoamide dehydrogenase, using the Rotor-Gene platform.

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Development of a rotor-gene real-time PCR assay for the detection and quantification ofMycoplasma genitalium