Characterization of BARD1 targeting and dynamics at the centrosome: The role of CRM1, BRCA1 and the Q564H mutation

Publication year: 2011 Source: Cellular Signalling, Available online 1 October 2011 Kirsty M. Brodie, Myth T.S. Mok, Beric R. Henderson BARD1 heterodimerizes with BRCA1, forming an E3 ubiquitin ligase that functions at nuclear foci to repair DNA damage and the centrosome to regulate mitosis. We compared BARD1 recruitment at these structures using fluorescence recovery after photobleaching assays to measure YFP-BARD1 dynamics in live cells. In nuclei at ionizing radiation-induced foci, 20% of the BARD1 pool was immobile and 80% of slow mobility exhibiting a recovery time >  500 s.

Publication year: 2011 Source: Cellular Signalling, Available online 1 October 2011 Kirsty M. Brodie, Myth T.S. Mok, Beric R. Henderson BARD1 heterodimerizes with BRCA1, forming an E3 ubiquitin ligase that functions at nuclear foci to repair DNA damage and the centrosome to regulate mitosis. We compared BARD1 recruitment at these structures using fluorescence recovery after photobleaching assays to measure YFP-BARD1 dynamics in live cells. In nuclei at ionizing radiation-induced foci, 20% of the BARD1 pool was immobile and 80% of slow mobility exhibiting a recovery time >  500 s.

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Characterization of BARD1 targeting and dynamics at the centrosome: The role of CRM1, BRCA1 and the Q564H mutation

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