Publication year: 2011 Source: Tissue and Cell, Available online 26 August 2011 Elisabetta Polazzi, Lorenzo Alibardi An in vitro system of isolated skin cells has been developed in order to address the understanding on the factors that control the shedding cycle and differentiation of lizard epidermis. The skin from the regenerating lizard tail has been separated in epidermis and dermis, cells have been dissociated, cultivated in vitro, and studied ultrastructurally after 1–30 days of culture condition. Dissociated keratinocytes after 12 days in culture show numerous cell elongations and contain bundles of keratin or sparse keratin filaments.
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Cell culture from lizard skin: A tool for the study of epidermal differentiation